Dendritic cells and toll-like receptors 2 and 4 in the ileum of Crohn's disease patients

Manuel A. Silva, Rodrigo Quera, Jorge Valenzuela, Sa'ad Y. Salim, Johan D. Söderholm, Mary H. Perdue

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We investigated myeloid-dendritic cell (DC) marker and Toll-like receptor (TLR)-2 and 4 distributions in ileal samples from Crohn's disease (CD) patients (n = 14) and controls (n = 13). In controls, no TLR-2+ cells were observed, and higher numbers of TLR-4+ and DC-SIGN+ cells (P < 0.01) were detected in ileal samples when compared versus colonic tissues. In non-inflamed CD ileum, TLR-4+ and DC-SGN+ cells were depleted from superficial areas of the villus, and a significant CD1a+ cell infiltration (P < 0.01) was observed when compared to ileal controls and non-inflamed colonic CD samples. In inflamed CD ileum, DC-SIGN+, CD1a+, TLR-4+ and few TLR-4 +DC-SIGN+ cells were detected as well as CD83 depletion. No correlation between TLR-2 and DC markers was detected in CD samples. A unique distribution of myeloid-DC markers characterized the CD ileum. Also, the presence of significant amounts of ileal CD1a+ cells may provide a relevant DC-mediated mechanism for antigen recognition in the pathogenesis of CD.

Original languageEnglish
Pages (from-to)1917-1928
Number of pages12
JournalDigestive Diseases and Sciences
Volume53
Issue number7
DOIs
StatePublished - Jul 2008
Externally publishedYes

Keywords

  • CD1a
  • Crohn's disease
  • Dendritic cells
  • Inflammation
  • Toll-like receptor

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