Deficient Vitamin E Uptake during Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI

Nicolás Santander; Carlos Lizama; María José Parga; Alonso Quiroz; Druso Pérez; Guadalupe Echeverría; Lorena Ulloa; Verónica Palma; Attilio Rigotti; Dolores Busso

doi:10.1038/s41598-017-05422-w

Deficient Vitamin E Uptake during Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI

Nicolás Santander, Carlos Lizama, María José Parga, Alonso Quiroz, Druso Pérez, Guadalupe Echeverría, Lorena Ulloa, Verónica Palma, Attilio Rigotti, Dolores Busso^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and Vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI^-/- embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic Vitamin E uptake during murine neural tube closure. Our results showed that SR-BI^-/- embryos had a very low Vitamin E content in comparison to SR-BI^+/+ embryos. Whereas SR-BI^-/- embryos with closed neural tubes (nSR-BI^-/-) had high levels of reactive oxygen species (ROS), intermediate ROS levels between SR-BI^+/+ and nSR-BI^-/- embryos were detected in SR-BI^-/- with NTD (NTD SR-BI^-/-). Reduced expression of Pax3, Alx1 and Alx3 genes was found in NTD SR-BI^-/- embryos. Maternal α-tocopherol dietary supplementation prevented NTD almost completely (from 54% to 2%, p < 0.001) in SR-BI^-/- embryos and normalized ROS and gene expression levels. In sum, our results suggest the involvement of SR-BI in the maternal provision of embryonic Vitamin E to the mouse embryo during neural tube closure.

Original language	English
Article number	5182
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-05422-w
State	Published - 1 Dec 2017
Externally published	Yes

Bibliographical note

Funding Information:
This study was financed by the Chilean National Council for Scientific and Technological Research (CONICYT) programs Fondo Nacional del Desarrollo Cient?fico y Tecnol?gico (FONDECYT) no. 1141236 (to D.B.), no. 1150399 (to A.R.), no. 1140697 (to V.P.), Ph.D. Fellowship no. 21130444 (to N.S.) and the School of Medicine grant PMD-04/16 (to N.S.).

Publisher Copyright:
© 2017 The Author(s).

Keywords

alpha-Tocopherol
Animals
Biomarkers
CD36 Antigens
Dietary Supplements
Embryonic Development
Gene Expression Regulation, Developmental
Mice
Mice, Knockout
Neural Tube
Oxidation-Reduction
Vitamin E
Yolk Sac

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@article{37b226da97e548339016b7df318a1dcb,

title = "Deficient Vitamin E Uptake during Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI",

abstract = "SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and Vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI-/- embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic Vitamin E uptake during murine neural tube closure. Our results showed that SR-BI-/- embryos had a very low Vitamin E content in comparison to SR-BI+/+ embryos. Whereas SR-BI-/- embryos with closed neural tubes (nSR-BI-/-) had high levels of reactive oxygen species (ROS), intermediate ROS levels between SR-BI+/+ and nSR-BI-/- embryos were detected in SR-BI-/- with NTD (NTD SR-BI-/-). Reduced expression of Pax3, Alx1 and Alx3 genes was found in NTD SR-BI-/- embryos. Maternal α-tocopherol dietary supplementation prevented NTD almost completely (from 54% to 2%, p < 0.001) in SR-BI-/- embryos and normalized ROS and gene expression levels. In sum, our results suggest the involvement of SR-BI in the maternal provision of embryonic Vitamin E to the mouse embryo during neural tube closure.",

keywords = "alpha-Tocopherol, Animals, Biomarkers, CD36 Antigens, Dietary Supplements, Embryonic Development, Gene Expression Regulation, Developmental, Mice, Mice, Knockout, Neural Tube, Oxidation-Reduction, Vitamin E, Yolk Sac",

author = "Nicol{\'a}s Santander and Carlos Lizama and Parga, {Mar{\'i}a Jos{\'e}} and Alonso Quiroz and Druso P{\'e}rez and Guadalupe Echeverr{\'i}a and Lorena Ulloa and Ver{\'o}nica Palma and Attilio Rigotti and Dolores Busso",

note = "Funding Information: This study was financed by the Chilean National Council for Scientific and Technological Research (CONICYT) programs Fondo Nacional del Desarrollo Cient?fico y Tecnol?gico (FONDECYT) no. 1141236 (to D.B.), no. 1150399 (to A.R.), no. 1140697 (to V.P.), Ph.D. Fellowship no. 21130444 (to N.S.) and the School of Medicine grant PMD-04/16 (to N.S.). Publisher Copyright: {\textcopyright} 2017 The Author(s).",

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doi = "10.1038/s41598-017-05422-w",

language = "English",

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T1 - Deficient Vitamin E Uptake during Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI

AU - Santander, Nicolás

AU - Lizama, Carlos

AU - Parga, María José

AU - Quiroz, Alonso

AU - Pérez, Druso

AU - Echeverría, Guadalupe

AU - Ulloa, Lorena

AU - Palma, Verónica

AU - Rigotti, Attilio

AU - Busso, Dolores

N1 - Funding Information: This study was financed by the Chilean National Council for Scientific and Technological Research (CONICYT) programs Fondo Nacional del Desarrollo Cient?fico y Tecnol?gico (FONDECYT) no. 1141236 (to D.B.), no. 1150399 (to A.R.), no. 1140697 (to V.P.), Ph.D. Fellowship no. 21130444 (to N.S.) and the School of Medicine grant PMD-04/16 (to N.S.). Publisher Copyright: © 2017 The Author(s).

PY - 2017/12/1

Y1 - 2017/12/1

N2 - SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and Vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI-/- embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic Vitamin E uptake during murine neural tube closure. Our results showed that SR-BI-/- embryos had a very low Vitamin E content in comparison to SR-BI+/+ embryos. Whereas SR-BI-/- embryos with closed neural tubes (nSR-BI-/-) had high levels of reactive oxygen species (ROS), intermediate ROS levels between SR-BI+/+ and nSR-BI-/- embryos were detected in SR-BI-/- with NTD (NTD SR-BI-/-). Reduced expression of Pax3, Alx1 and Alx3 genes was found in NTD SR-BI-/- embryos. Maternal α-tocopherol dietary supplementation prevented NTD almost completely (from 54% to 2%, p < 0.001) in SR-BI-/- embryos and normalized ROS and gene expression levels. In sum, our results suggest the involvement of SR-BI in the maternal provision of embryonic Vitamin E to the mouse embryo during neural tube closure.

AB - SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and Vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI-/- embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic Vitamin E uptake during murine neural tube closure. Our results showed that SR-BI-/- embryos had a very low Vitamin E content in comparison to SR-BI+/+ embryos. Whereas SR-BI-/- embryos with closed neural tubes (nSR-BI-/-) had high levels of reactive oxygen species (ROS), intermediate ROS levels between SR-BI+/+ and nSR-BI-/- embryos were detected in SR-BI-/- with NTD (NTD SR-BI-/-). Reduced expression of Pax3, Alx1 and Alx3 genes was found in NTD SR-BI-/- embryos. Maternal α-tocopherol dietary supplementation prevented NTD almost completely (from 54% to 2%, p < 0.001) in SR-BI-/- embryos and normalized ROS and gene expression levels. In sum, our results suggest the involvement of SR-BI in the maternal provision of embryonic Vitamin E to the mouse embryo during neural tube closure.

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KW - Biomarkers

KW - CD36 Antigens

KW - Dietary Supplements

KW - Embryonic Development

KW - Gene Expression Regulation, Developmental

KW - Mice

KW - Mice, Knockout

KW - Neural Tube

KW - Oxidation-Reduction

KW - Vitamin E

KW - Yolk Sac

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DO - 10.1038/s41598-017-05422-w

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C2 - 28701710

AN - SCOPUS:85024106647

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 5182

ER -

Deficient Vitamin E Uptake during Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI

Abstract

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Keywords

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