Creation of an amino acid network of structurally coupled residues in the directed evolution of a thermostable enzyme

Manfred T. Reetz; Pankaj Soni; Juan Pablo Acevedo; Joaquin Sanchis

doi:10.1002/anie.200904209

Creation of an amino acid network of structurally coupled residues in the directed evolution of a thermostable enzyme

Manfred T. Reetz, Pankaj Soni, Juan Pablo Acevedo, Joaquin Sanchis

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Molecular orchestration: In the directed evolution of a hyperthermally stabilized enzyme, remote residues have been structurally coupled as a result of the creation of an extensive communicating amino acid network on the surface of the protein.

Original language	English
Pages (from-to)	8268-8272
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	48
Issue number	44
DOIs	https://doi.org/10.1002/anie.200904209
State	Published - 19 Oct 2009
Externally published	Yes

Keywords

Directed evolution
Enzymes
Hydrogen bonds
Protein modifications
Saturation mutagenesis

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10.1002/anie.200904209

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title = "Creation of an amino acid network of structurally coupled residues in the directed evolution of a thermostable enzyme",

abstract = "Molecular orchestration: In the directed evolution of a hyperthermally stabilized enzyme, remote residues have been structurally coupled as a result of the creation of an extensive communicating amino acid network on the surface of the protein.",

keywords = "Directed evolution, Enzymes, Hydrogen bonds, Protein modifications, Saturation mutagenesis",

author = "Reetz, \{Manfred T.\} and Pankaj Soni and Acevedo, \{Juan Pablo\} and Joaquin Sanchis",

year = "2009",

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doi = "10.1002/anie.200904209",

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AU - Reetz, Manfred T.

AU - Soni, Pankaj

AU - Acevedo, Juan Pablo

AU - Sanchis, Joaquin

PY - 2009/10/19

Y1 - 2009/10/19

N2 - Molecular orchestration: In the directed evolution of a hyperthermally stabilized enzyme, remote residues have been structurally coupled as a result of the creation of an extensive communicating amino acid network on the surface of the protein.

AB - Molecular orchestration: In the directed evolution of a hyperthermally stabilized enzyme, remote residues have been structurally coupled as a result of the creation of an extensive communicating amino acid network on the surface of the protein.

KW - Directed evolution

KW - Enzymes

KW - Hydrogen bonds

KW - Protein modifications

KW - Saturation mutagenesis

UR - https://www.scopus.com/pages/publications/70350110646

U2 - 10.1002/anie.200904209

DO - 10.1002/anie.200904209

M3 - Article

C2 - 19787674

AN - SCOPUS:70350110646

SN - 1433-7851

VL - 48

SP - 8268

EP - 8272

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 44

ER -

Creation of an amino acid network of structurally coupled residues in the directed evolution of a thermostable enzyme

Abstract

Keywords

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