Creation of an amino acid network of structurally coupled residues in the directed evolution of a thermostable enzyme

Manfred T. Reetz, Pankaj Soni, Juan Pablo Acevedo, Joaquin Sanchis

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Molecular orchestration: In the directed evolution of a hyperthermally stabilized enzyme, remote residues have been structurally coupled as a result of the creation of an extensive communicating amino acid network on the surface of the protein.

Original languageEnglish
Pages (from-to)8268-8272
Number of pages5
JournalAngewandte Chemie - International Edition
Volume48
Issue number44
DOIs
StatePublished - 19 Oct 2009
Externally publishedYes

Keywords

  • Directed evolution
  • Enzymes
  • Hydrogen bonds
  • Protein modifications
  • Saturation mutagenesis

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