Combining autologous particulate dentin, L-PRF, and fibrinogen to create a matrix for predictable ridge preservation: a pilot clinical study

Catherine Andrade*, Joaquin Camino, Mauricio Nally, Marc Quirynen, Benjamín Martínez, Nelson Pinto

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Objectives: The aim of this study was to describe the histological and clinical outcome of “dentin block” (a mixture of autologous particulate dentin, leukocyte- and platelet-rich fibrin (L-PRF), and liquid fibrinogen) in alveolar ridge preservation. Material and methods: Ten extraction sockets were grafted with “dentin block,” a mixture of particulate autologous dentin with chopped leukocyte-platelet-rich fibrin (L-PRF) membranes at a 1:1 ratio, and liquid fibrinogen as a binder. Two grafted sites were followed at 4 and 5 months, and 6 sites at 6 months. Biopsies were taken from the core of the grafted site for histologic and histo-morphometric analysis. Results: All patients completed the study without any adverse event. The vertical and horizontal dimensions of the alveolar ridge were preserved or even increased after 4, 5, or 6 months and remained stable after 6 months of the implant placement. The histological examination revealed a median relative percentage of bone, dentin, and connective tissue of 57.0, 0.9, and 39.3%, respectively. A comparison of samples at different time points (4, 5, and 6 months) showed a progressive increase in the proportion of bone with a decrease in the proportion of dentin. The bone was compact with normal osteocytes and moderate osteoblastic activity. In 4 out of 10 samples, no dentin was observed; in the other samples, it represented 1–5% (with geometric fragments). Conclusions: Dentin block showed to be a suitable bone substitute in an alveolar ridges preservation model. Clinical relevance: The promising results of dentin block as a bone substitute in alveolar ridge preservation could have an important clinical impact considering this biomaterial brings together the regenerative potential of three autologous products with excellent biological and clinical behavior, low risk of adverse effects, and feasible acquisition.

Original languageEnglish
Pages (from-to)1151-1160
Number of pages10
JournalClinical Oral Investigations
Issue number3
StatePublished - 1 Mar 2020

Bibliographical note

Funding Information:
The present study was supported by own funds for research projects of Universidad de Los Andes. Acknowledgments

Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.


  • Bone regeneration
  • Bone substitute
  • Dentin
  • Dentin block
  • GBR
  • Ridge preservation


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