Characterization of the petI and res operons of Acidithiobacillus ferrooxidans

Gloria Levicán, Patrice Bruscella, Maritza Guacunano, Carolina Inostroza Silva, Violaine Bonnefoy, David S. Holmes, Eugenia Jedlicki

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38 Scopus citations

Abstract

DNA sequence analysis and bioinformatic interpretations have identified two adjacent clusters of genes potentially involved in the formation of a bc1 complex and in the maturation of a cytochrome c-type protein in two strains (ATCC 19859 and ATCC 33020) of the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans). Reverse transcriptase-PCR experiments suggest that the two clusters are organized as operons, and +1 start sites of transcription for the operons have been determined by primer extension experiments. Potential promoters have been identified. The presence of these operons lends support to a recent model of reverse electron flow and is consistent with previous reports of phenotypic switching in this bacterium.

Original languageEnglish
Pages (from-to)1498-1501
Number of pages4
JournalJournal of Bacteriology
Volume184
Issue number5
DOIs
StatePublished - 2002
Externally publishedYes

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