Characterization of progressive periodontal lesions in chronic periodontitis patients: Levels of chemokines, cytokines, matrix metalloproteinase-13, periodontal pathogens and inflammatory cells

Nora Silva*, Nicolas Dutzan, Marcela Hernandez, Andrea Dezerega, Oriana Rivera, Juan Carlos Aguillon, Octavio Aravena, Pedro Lastres, Patricia Pozo, Rolando Vernal, Jorge Gamonal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

Background and aims: Periodontitis is an infection with an episodic nature of tissue support destruction. The aim of this work was to determine the levels of chemokines, cytokines, matrix metalloproteinase-13, periodontal pathogens and inflammatory cells in periodontal sites characterized by active periodontal connective tissue destruction. Material and Method: Fifty-six patients with moderate or advanced severity of chronic periodontitis were selected. Periodontitis was characterized by at least six sites with probing depth ≥5 mm, clinical attachment level ≥3 mm and radiographic bone loss. Periodontitis progression was determined by the tolerance method. Receptor activator for nuclear factor κ B-ligand (RANK-L), monocyte chemoattractant protein-1 (MCP-1), tumour necrosis factor-α (TNF-α), IL-1β, MMP-13, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsithia and inflammatory cells levels were determined. Statistical analysis was performed using the Stata® 7.0 software. Data were expressed as mean±SD and paired samples t-test and χ2 tests were used. Results: Higher RANK-L, IL-1β and MMP-13 activity levels were observed in active sites (p<0.05). The proportion of P. gingivalis, A. actinomycetemcomitans, T. forsythia and the number of CD4+ T were higher in active than in inactive sites (p>0.05). Conclusion: The detection of periodontopathic bacteria, host matrix metalloproteinases and cytokines in periodontitis patients with lesions undergoing episodic attachment loss could partially explain the mechanisms associated with the destruction of the supporting tissues of the tooth.

Original languageEnglish
Pages (from-to)206-214
Number of pages9
JournalJournal of Clinical Periodontology
Volume35
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Keywords

  • Cytokines
  • Inflammatory cells
  • Matrix metalloproteinases
  • Periodontal pathogens
  • Progressive periodontitis

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