Characterization of a Gelatin/chitosan/hyaluronan scaffold-polymer

Javier Enrione; Fernando Osorio; Daniel López; Caroline Weinstein-Oppenheimer; Miguel A. Fuentes; Ricardo Ceriani; Donald I. Brown; Fernando Albornoz; Elizabeth Sánchez; Patricio Villalobos; Rodrigo A. Somoza; Manuel E. Young; Cristian A. Acevedo

doi:10.2225/vol13-issue5-fulltext-15

Characterization of a Gelatin/chitosan/hyaluronan scaffold-polymer

Javier Enrione, Fernando Osorio, Daniel López, Caroline Weinstein-Oppenheimer, Miguel A. Fuentes, Ricardo Ceriani, Donald I. Brown, Fernando Albornoz, Elizabeth Sánchez, Patricio Villalobos, Rodrigo A. Somoza, Manuel E. Young, Cristian A. Acevedo

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30° could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin, reporting biological behaviour and its mechanical properties.

Original language	English
Journal	Electronic Journal of Biotechnology
Volume	13
Issue number	5
DOIs	https://doi.org/10.2225/vol13-issue5-fulltext-15
State	Published - 2010
Externally published	Yes

Keywords

Chitosan
Fibrin
Gelatin
Hyaluronic acid
Scaffold

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10.2225/vol13-issue5-fulltext-15

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title = "Characterization of a Gelatin/chitosan/hyaluronan scaffold-polymer",

abstract = "Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30° could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin, reporting biological behaviour and its mechanical properties.",

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language = "English",

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T1 - Characterization of a Gelatin/chitosan/hyaluronan scaffold-polymer

AU - Enrione, Javier

AU - Osorio, Fernando

AU - López, Daniel

AU - Weinstein-Oppenheimer, Caroline

AU - Fuentes, Miguel A.

AU - Ceriani, Ricardo

AU - Brown, Donald I.

AU - Albornoz, Fernando

AU - Sánchez, Elizabeth

AU - Villalobos, Patricio

AU - Somoza, Rodrigo A.

AU - Young, Manuel E.

AU - Acevedo, Cristian A.

PY - 2010

Y1 - 2010

N2 - Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30° could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin, reporting biological behaviour and its mechanical properties.

AB - Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30° could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin, reporting biological behaviour and its mechanical properties.

KW - Chitosan

KW - Fibrin

KW - Gelatin

KW - Hyaluronic acid

KW - Scaffold

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U2 - 10.2225/vol13-issue5-fulltext-15

DO - 10.2225/vol13-issue5-fulltext-15

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SN - 0717-3458

VL - 13

JO - Electronic Journal of Biotechnology

JF - Electronic Journal of Biotechnology

IS - 5

ER -

Characterization of a Gelatin/chitosan/hyaluronan scaffold-polymer

Abstract

Keywords

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