In the crab Chasmagnathus learning model, systemic administration of cAMP analogues that are specific activators or inhibitors of cAMP-dependent protein kinase (PKA) proved to respectively facilitate or impair long-term retention. The aims of the present work were to analyse PKA activity distribution in the crab brain and to characterise PKA isoforms. The neuropils from the eyestalk showed higher levels of induced PKA activity when compared with other neuropils of the central nervous system. Two PKA isoforms, homologous to mammalian PKA I and PKA II, were detected from central brain protein extracts using DEAE chromatography. Only PKA II was found in lateral protocerebrum extracts, suggesting a role of this isoform in the processing of visual inputs and in the integration of this information with other sensory inputs. PKA I was observed to be ten-fold more sensitive to cAMP than PKA II. cGMP induced a high activation of both PKA isoforms, similar to that obtained with cAMP. PKA I showed a two-fold greater sensitivity for cGMP than PKA II. An autophosphorylation assay was performed and a protein of 55 kDa, corresponding to phosphorylated R II regulatory subunit, was detected. The presence of a PKA I isoform with high sensitivity for cAMP in the central brain suggests a role of this subtype in long-term memory.
|Number of pages||8|
|Journal||Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology|
|State||Published - 2001|
Bibliographical noteFunding Information:
Acknowledgements We thank Dr. Cristina Pavetto and Dr. Liliana Orelli. This work was supported by Fundación Antorchas and University of Buenos Aires grants. All the experiments performed in this work comply with the current laws of Argentina.
- cAMP and cGMP
- Crab brain
- PKA autophosphorylation
- PKA isoforms