TY - JOUR
T1 - C-reactive protein expression is up-regulated in apical lesions of endodontic origin in association with interleukin-6
AU - Garrido, Mauricio
AU - Dezerega, Andrea
AU - Bordagaray, María José
AU - Reyes, Montserrat
AU - Vernal, Rolando
AU - Melgar-Rodríguez, Samantha
AU - Ciuchi, Pía
AU - Paredes, Rodolfo
AU - García-Sesnich, Jocelyn
AU - Ahumada-Montalva, Pablo
AU - Hernández, Marcela
N1 - Publisher Copyright:
Copyright © 2015 American Association of Endodontists.
PY - 2015
Y1 - 2015
N2 - Introduction: C-reactive protein (CRP) is the prototype component of acute-phase proteins induced ultimately by interleukin (IL)-6 in the liver, but it is unknown whether periradicular tissues locally express CRP. The present study aimed to identify whether CRP messenger RNA synthesis occurs in situ within apical lesions of endodontic origin (ALEOs) and healthy periodontal ligament and its association with IL-6 and to determine their protein levels and tissue localization. Methods: Patients with asymptomatic apical periodontitis and healthy volunteers presenting at the School of Dentistry, University of Chile, Santiago, Chile, were enrolled. ALEOs and healthy teeth were obtained and processed for either immunohistochemistry and double immunofluorescence to assess IL-6 and CRP tissue localization, whereas healthy periodontal ligaments were processed as controls for real-time reverse-transcription polymerase chain reaction for their RNA expression levels and multiplex assay to determine their protein levels. Statistic analysis was performed using the unpaired t test or Mann- Whitney test according to data distribution and Pearson correlation. Results: IL-6 and CRP were synthesized in ALEOs, whereas their RNA expression and protein levels were significantly higher when compared with healthy periodontal ligament. IL-6 and CRP immunolocalized to the inflammatory cells, vascular endothelial cells, and mesenchymal cells. Both, IL-6 and CRP colocalized in ALEOs, and a positive correlation was found between their expression levels (P < .05). Conclusions: IL-6 and CRP messenger RNA are constitutively expressed in periodontal ligament and upregulated in ALEOs along with higher protein levels. Given their pleiotropic effects, IL-6 and CRP protein levels in apical tissues might partially explain the development and progression of ALEOs as well as potentially asymptomatic apical periodontitis-associated systemic low-grade inflammation.
AB - Introduction: C-reactive protein (CRP) is the prototype component of acute-phase proteins induced ultimately by interleukin (IL)-6 in the liver, but it is unknown whether periradicular tissues locally express CRP. The present study aimed to identify whether CRP messenger RNA synthesis occurs in situ within apical lesions of endodontic origin (ALEOs) and healthy periodontal ligament and its association with IL-6 and to determine their protein levels and tissue localization. Methods: Patients with asymptomatic apical periodontitis and healthy volunteers presenting at the School of Dentistry, University of Chile, Santiago, Chile, were enrolled. ALEOs and healthy teeth were obtained and processed for either immunohistochemistry and double immunofluorescence to assess IL-6 and CRP tissue localization, whereas healthy periodontal ligaments were processed as controls for real-time reverse-transcription polymerase chain reaction for their RNA expression levels and multiplex assay to determine their protein levels. Statistic analysis was performed using the unpaired t test or Mann- Whitney test according to data distribution and Pearson correlation. Results: IL-6 and CRP were synthesized in ALEOs, whereas their RNA expression and protein levels were significantly higher when compared with healthy periodontal ligament. IL-6 and CRP immunolocalized to the inflammatory cells, vascular endothelial cells, and mesenchymal cells. Both, IL-6 and CRP colocalized in ALEOs, and a positive correlation was found between their expression levels (P < .05). Conclusions: IL-6 and CRP messenger RNA are constitutively expressed in periodontal ligament and upregulated in ALEOs along with higher protein levels. Given their pleiotropic effects, IL-6 and CRP protein levels in apical tissues might partially explain the development and progression of ALEOs as well as potentially asymptomatic apical periodontitis-associated systemic low-grade inflammation.
KW - Apical periodontitis
KW - C-reactive protein
KW - Interleukin-6
UR - http://www.scopus.com/inward/record.url?scp=84933178518&partnerID=8YFLogxK
U2 - 10.1016/j.joen.2014.12.021
DO - 10.1016/j.joen.2014.12.021
M3 - Article
C2 - 25748492
AN - SCOPUS:84933178518
SN - 0099-2399
VL - 41
SP - 464
EP - 469
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 4
ER -