Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles

Mario F. Gutiérrez; Luisa F. Alegría-Acevedo; Luján Méndez-Bauer; Jorge Bermudez; Andrés Dávila-Sánchez; Sonja Buvinic; Nadia Hernández-Moya; Alessandra Reis; Alessandro D. Loguercio; Paulo V. Farago; Javier Martin; Eduardo Fernández

doi:10.1016/j.jdent.2019.01.012

Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles

Mario F. Gutiérrez, Luisa F. Alegría-Acevedo, Luján Méndez-Bauer, Jorge Bermudez, Andrés Dávila-Sánchez, Sonja Buvinic, Nadia Hernández-Moya, Alessandra Reis, Alessandro D. Loguercio, Paulo V. Farago, Javier Martin, Eduardo Fernández^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Objectives: To evaluate the effect of addition of zinc oxide and copper nanoparticles (ZnO/CuNp) into universal adhesives, on antimicrobial activity (AMA), cytotoxicity (CTX), water sorption (WS) and solubility (SO), microhardness (MH) and in vitro degree of conversion (DC), as well as resin-dentin microtensile bond strength (μTBS), nanoleakage (NL) and in situ DC. Methods: ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added in Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans. For CTX, Saos-2 cell-line was used. For WS and SO, specimens were tested for 28d. For MH, specimens were tested after 24 h and 28d and for in vitro DC, specimens were evaluated after 24 h. After, the adhesives were applied to flat dentine surfaces, composite resin build-ups, specimens were sectioned to obtain resin–dentine sticks. It was evaluated in μTBS, NL and in situ DC after 24 h of water storage. ANOVA and Tukey's test were applied (α = 0.05). Results: The addition of 5/0.2 ZnO/CuNp increase AMA and WS, but decrease the SO when compared to control (p < 0.05). The CTX and μTBS were maintaining with adhesive-containing ZnO/CuNp (p > 0.05). MH, in vitro DC and in situ DC was significant increase (AMB) or maintaining (PBA) with ZnO/CuNp addition. However, significantly lower NL was observed for ZnO/CuNp groups (p < 0.05). Conclusions: The addition of ZnO/CuNp in the tested concentrations in universal adhesive systems may be an alternative to provide antimicrobial activity and improves the integrity of the hybrid layer, without jeopardizing biological, adhesives and mechanical properties. Significance: This is the first study that demonstrates that the addition of zinc oxide and copper nanoparticles in concentrations up to 5/0.2 wt% in two universal adhesive systems is a feasible approach and may be an alternative to adhesive interfaces with antimicrobial properties and less defects in the resin-dentin interface.

Original language	English
Pages (from-to)	45-55
Number of pages	11
Journal	Journal of Dentistry
Volume	82
DOIs	https://doi.org/10.1016/j.jdent.2019.01.012
State	Published - Mar 2019
Externally published	Yes

Bibliographical note

Funding Information:
This study was performed by Mario Felipe Guti?rrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001. The corresponding author dedicates this article to his two kids, Elisa and Eduardo, for their enormous inspiration.

Funding Information:
This study was performed by Mario Felipe Gutiérrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Científico y Tecnológico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The corresponding author dedicates this article to his two kids, Elisa and Eduardo, for their enormous inspiration.

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

Copper
Microtensile bond strength and nanoleakage
Nanoparticles
Universal adhesive system
Zinc oxide

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10.1016/j.jdent.2019.01.012

Cite this

Gutiérrez, M. F., Alegría-Acevedo, L. F., Méndez-Bauer, L., Bermudez, J., Dávila-Sánchez, A., Buvinic, S., Hernández-Moya, N., Reis, A., Loguercio, A. D., Farago, P. V., Martin, J., & Fernández, E. (2019). Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles. Journal of Dentistry, 82, 45-55. https://doi.org/10.1016/j.jdent.2019.01.012

@article{838fba036dab4bc5928077a836347caa,

title = "Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles",

abstract = "Objectives: To evaluate the effect of addition of zinc oxide and copper nanoparticles (ZnO/CuNp) into universal adhesives, on antimicrobial activity (AMA), cytotoxicity (CTX), water sorption (WS) and solubility (SO), microhardness (MH) and in vitro degree of conversion (DC), as well as resin-dentin microtensile bond strength (μTBS), nanoleakage (NL) and in situ DC. Methods: ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added in Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans. For CTX, Saos-2 cell-line was used. For WS and SO, specimens were tested for 28d. For MH, specimens were tested after 24 h and 28d and for in vitro DC, specimens were evaluated after 24 h. After, the adhesives were applied to flat dentine surfaces, composite resin build-ups, specimens were sectioned to obtain resin–dentine sticks. It was evaluated in μTBS, NL and in situ DC after 24 h of water storage. ANOVA and Tukey's test were applied (α = 0.05). Results: The addition of 5/0.2 ZnO/CuNp increase AMA and WS, but decrease the SO when compared to control (p < 0.05). The CTX and μTBS were maintaining with adhesive-containing ZnO/CuNp (p > 0.05). MH, in vitro DC and in situ DC was significant increase (AMB) or maintaining (PBA) with ZnO/CuNp addition. However, significantly lower NL was observed for ZnO/CuNp groups (p < 0.05). Conclusions: The addition of ZnO/CuNp in the tested concentrations in universal adhesive systems may be an alternative to provide antimicrobial activity and improves the integrity of the hybrid layer, without jeopardizing biological, adhesives and mechanical properties. Significance: This is the first study that demonstrates that the addition of zinc oxide and copper nanoparticles in concentrations up to 5/0.2 wt% in two universal adhesive systems is a feasible approach and may be an alternative to adhesive interfaces with antimicrobial properties and less defects in the resin-dentin interface.",

keywords = "Copper, Microtensile bond strength and nanoleakage, Nanoparticles, Universal adhesive system, Zinc oxide",

author = "Guti{\'e}rrez, {Mario F.} and Alegr{\'i}a-Acevedo, {Luisa F.} and Luj{\'a}n M{\'e}ndez-Bauer and Jorge Bermudez and Andr{\'e}s D{\'a}vila-S{\'a}nchez and Sonja Buvinic and Nadia Hern{\'a}ndez-Moya and Alessandra Reis and Loguercio, {Alessandro D.} and Farago, {Paulo V.} and Javier Martin and Eduardo Fern{\'a}ndez",

note = "Funding Information: This study was performed by Mario Felipe Guti?rrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001. The corresponding author dedicates this article to his two kids, Elisa and Eduardo, for their enormous inspiration. Funding Information: This study was performed by Mario Felipe Guti{\'e}rrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Cient{\'i}fico y Tecnol{\'o}gico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior - Brasil (CAPES) - Finance Code 001. The corresponding author dedicates this article to his two kids, Elisa and Eduardo, for their enormous inspiration. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = mar,

doi = "10.1016/j.jdent.2019.01.012",

language = "English",

volume = "82",

pages = "45--55",

journal = "Journal of Dentistry",

issn = "0300-5712",

publisher = "Elsevier BV",

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Gutiérrez, MF, Alegría-Acevedo, LF, Méndez-Bauer, L, Bermudez, J, Dávila-Sánchez, A, Buvinic, S, Hernández-Moya, N, Reis, A, Loguercio, AD, Farago, PV, Martin, J & Fernández, E 2019, 'Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles', Journal of Dentistry, vol. 82, pp. 45-55. https://doi.org/10.1016/j.jdent.2019.01.012

TY - JOUR

T1 - Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles

AU - Gutiérrez, Mario F.

AU - Alegría-Acevedo, Luisa F.

AU - Méndez-Bauer, Luján

AU - Bermudez, Jorge

AU - Dávila-Sánchez, Andrés

AU - Buvinic, Sonja

AU - Hernández-Moya, Nadia

AU - Reis, Alessandra

AU - Loguercio, Alessandro D.

AU - Farago, Paulo V.

AU - Martin, Javier

AU - Fernández, Eduardo

N1 - Funding Information: This study was performed by Mario Felipe Guti?rrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001. The corresponding author dedicates this article to his two kids, Elisa and Eduardo, for their enormous inspiration. Funding Information: This study was performed by Mario Felipe Gutiérrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Científico y Tecnológico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The corresponding author dedicates this article to his two kids, Elisa and Eduardo, for their enormous inspiration. Publisher Copyright: © 2019 Elsevier Ltd

PY - 2019/3

Y1 - 2019/3

N2 - Objectives: To evaluate the effect of addition of zinc oxide and copper nanoparticles (ZnO/CuNp) into universal adhesives, on antimicrobial activity (AMA), cytotoxicity (CTX), water sorption (WS) and solubility (SO), microhardness (MH) and in vitro degree of conversion (DC), as well as resin-dentin microtensile bond strength (μTBS), nanoleakage (NL) and in situ DC. Methods: ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added in Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans. For CTX, Saos-2 cell-line was used. For WS and SO, specimens were tested for 28d. For MH, specimens were tested after 24 h and 28d and for in vitro DC, specimens were evaluated after 24 h. After, the adhesives were applied to flat dentine surfaces, composite resin build-ups, specimens were sectioned to obtain resin–dentine sticks. It was evaluated in μTBS, NL and in situ DC after 24 h of water storage. ANOVA and Tukey's test were applied (α = 0.05). Results: The addition of 5/0.2 ZnO/CuNp increase AMA and WS, but decrease the SO when compared to control (p < 0.05). The CTX and μTBS were maintaining with adhesive-containing ZnO/CuNp (p > 0.05). MH, in vitro DC and in situ DC was significant increase (AMB) or maintaining (PBA) with ZnO/CuNp addition. However, significantly lower NL was observed for ZnO/CuNp groups (p < 0.05). Conclusions: The addition of ZnO/CuNp in the tested concentrations in universal adhesive systems may be an alternative to provide antimicrobial activity and improves the integrity of the hybrid layer, without jeopardizing biological, adhesives and mechanical properties. Significance: This is the first study that demonstrates that the addition of zinc oxide and copper nanoparticles in concentrations up to 5/0.2 wt% in two universal adhesive systems is a feasible approach and may be an alternative to adhesive interfaces with antimicrobial properties and less defects in the resin-dentin interface.

AB - Objectives: To evaluate the effect of addition of zinc oxide and copper nanoparticles (ZnO/CuNp) into universal adhesives, on antimicrobial activity (AMA), cytotoxicity (CTX), water sorption (WS) and solubility (SO), microhardness (MH) and in vitro degree of conversion (DC), as well as resin-dentin microtensile bond strength (μTBS), nanoleakage (NL) and in situ DC. Methods: ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added in Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans. For CTX, Saos-2 cell-line was used. For WS and SO, specimens were tested for 28d. For MH, specimens were tested after 24 h and 28d and for in vitro DC, specimens were evaluated after 24 h. After, the adhesives were applied to flat dentine surfaces, composite resin build-ups, specimens were sectioned to obtain resin–dentine sticks. It was evaluated in μTBS, NL and in situ DC after 24 h of water storage. ANOVA and Tukey's test were applied (α = 0.05). Results: The addition of 5/0.2 ZnO/CuNp increase AMA and WS, but decrease the SO when compared to control (p < 0.05). The CTX and μTBS were maintaining with adhesive-containing ZnO/CuNp (p > 0.05). MH, in vitro DC and in situ DC was significant increase (AMB) or maintaining (PBA) with ZnO/CuNp addition. However, significantly lower NL was observed for ZnO/CuNp groups (p < 0.05). Conclusions: The addition of ZnO/CuNp in the tested concentrations in universal adhesive systems may be an alternative to provide antimicrobial activity and improves the integrity of the hybrid layer, without jeopardizing biological, adhesives and mechanical properties. Significance: This is the first study that demonstrates that the addition of zinc oxide and copper nanoparticles in concentrations up to 5/0.2 wt% in two universal adhesive systems is a feasible approach and may be an alternative to adhesive interfaces with antimicrobial properties and less defects in the resin-dentin interface.

KW - Copper

KW - Microtensile bond strength and nanoleakage

KW - Nanoparticles

KW - Universal adhesive system

KW - Zinc oxide

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DO - 10.1016/j.jdent.2019.01.012

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VL - 82

SP - 45

EP - 55

JO - Journal of Dentistry

JF - Journal of Dentistry

ER -

Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles

Abstract

Bibliographical note

Keywords

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