Biogas production from winery wastewater: Effect of the substrate-inoculum ratio on fly ash addition and iron availability

Yeney Lauzurique, Fernando G. Fermoso, Nicolás Sánchez, Alejandra Castillo, Ricardo Salazar, Verónica García, César Huiliñir*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The effect of substrate-inoculum ratio and fly ash (FA) concentration on the anaerobic digestion of winery wastewater was studied. Two substrate-inoculum ratios (0.50 and 1 g soluble COD/g VSS) and five fly ash concentrations (25, 50, 75, 100 and 150 mg/L) were tested under mesophilic conditions. The results showed that 0.50 g soluble COD/g VSS was the best substrate-inoculum ratio. Regarding the FA, 100 mg/L improved the specific methane production by 79% compared to the control assay. In comparison, 150 mg/L produced an inhibition of methane production, with a 55% decrease in specific methane production with respect to the control assay. According to the bioavailability study, FA had a similar effect to a system with Fe-EDTA on anaerobic digestion, which could indicate that the Fe present in FA was present in the solution and bioavailable to microorganisms. Therefore, a possible mechanism for the action of FA on anaerobic digestion was proposed. Finally, the kinetic analysis showed that FA improved the maximum specific methane production rate and decreased the lag phase.

Original languageEnglish
Article number102826
JournalJournal of Water Process Engineering
Volume47
DOIs
StatePublished - Jun 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

  • Bioavailability
  • Fly ash
  • Modified Gompertz model
  • Specific methane production
  • Substrate-inoculum ratio

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