Assessment of gelatin-chitosan interactions in films by a chemometrics approach

Cristian A. Acevedo, Paulo Diáz-Calderón, Daniel López, Javier Enrione*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Information looking at the interaction mechanism of chitosan-gelatin is scarce. The objective of this work was to analyze the interactions between chitosan and bovine (BG) and salmon gelatins using a chemometrics approach based on the multivariate statistical analysis, partial least square regression (PLSR), with data collected from amino acid profiling and differential scanning calorimetry.Gelatins showed differences in imino acid profile (higher amount of proline and hydroxyproline in BG). Chitosan modified the microstructure of films as indicated by variations in glass transition temperature (Tg), heat capacity (Cp), melting temperature (Tm), and enthalpy (H), in partially crystalline and amorphous films due to the effect of chitosan on gelatin chain renaturation and moisture content. PLSR showed that thermal properties of gelatin-chitosan films were affected by presence of amino acids Cysteine, Tyrosine, Valine, Arginine, Hydroxyproline, and Glycine, which would modify the orientation of side chains of polymer, while charged amino acids would encourage gelatin renaturation rather than gelatin-chitosan interaction.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalCYTA - Journal of Food
Issue number2
StatePublished - 3 Apr 2015

Bibliographical note

Publisher Copyright:
© 2014 Taylor and Francis.


  • Salmon gelatin
  • bovine gelatin
  • chitosan
  • multivariable statistical analysis
  • polymer interactions


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