Anatase incorporation to bioactive scaffolds based on salmon gelatin and its effects on muscle cell growth

Cristian A. Acevedo, Yusser Olguín, Nicole Orellana, Elizabeth Sánchez, Marzena Pepczynska, Javier Enrione*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The development of new polymer scaffolds is essential for tissue engineering and for culturing cells. The use of non-mammalian bioactive components to formulate these materials is an emerging field. In our previous work, a scaffold based on salmon gelatin was developed and tested in animal models to regenerate tissues effectively and safely. Here, the incorporation of anatase nanoparticles into this scaffold was formulated, studying the new composite structure by scanning electron microscopy, differential scanning calorimetry and dynamic mechanical analysis. The incorporation of anatase nanoparticles modified the scaffold microstructure by increasing the pore size from 208 to 239 μm and significantly changing the pore shape. The glass transition temperature changed from 46.9 to 55.8 °C, and an increase in the elastic modulus from 79.5 to 537.8 kPa was observed. The biocompatibility of the scaffolds was tested using C2C12 myoblasts, modulating their attachment and growth. The anatase nanoparticles modified the stiffness of the material, making it possible to increase the growth of myoblasts cultured onto scaffolds, which envisions their use in muscle tissue engineering.

Original languageEnglish
Article number1943
Issue number9
StatePublished - Sep 2020

Bibliographical note

Funding Information:
Funding: The authors would like to express thanks for the financial support from CONICYT-ANID (Chile), through the following grants: FONDECYT 1190100, PIA/APOYO AFB180002, and FONDECYT 1201584.

Publisher Copyright:
© 2020 by the authors.


  • Anatase
  • Muscle cells
  • Salmon gelatin
  • Scaffold
  • Tissue engineering


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