Analysis of enamel/restoration interface submitted cariogenic challenge and fluoride release

Raquel Viana Rodrigues, Camila Sobral Sampaio, Aline Carvalho Girotto, Caroline Paiuta Pinhatti, Alexsandra Shizue Iwamoto, Anderson Zanardi de Freitas, Gláucia Maria Bovi Ambrosano, Regina Maria Puppin-Rontani, Fernanda Miori Pascon

Research output: Contribution to journalArticlepeer-review

Abstract

The treatment of high-risk patients still is a challenge. The understanding and development non-invasive, non-destructive, and non-ionizing techniques, can help to guide the treatment and the diagnosis of primary and recurrent caries. The present study evaluated the behavior of enamel/restoration interface after a cariogenic challenge by Fourier domain optical coherence tomography (FD-OCT), scanning electron microscopy (SEM) examination, and the fluoride release of the different restorative materials. Cavities (1.5 × 0.5 mm) were performed in enamel surface and divided into groups (n = 8): glass ionomer cement (GIC), resin-modified glass ionomer cement (RMGIC), and resin composite (RC). The samples were submitted to pH-cycling, and the solutions analyzed for cumulative fluoride by ion-analyzer. The morphology was analyzed by SEM through replicas. The optical attenuation coefficient (OAC) was calculated through exponential decay from the images generated by FD-OCT. Data were analyzed considering α = 0.05. OAC values increased for all groups after pH-cycling indicating demineralization (p <.05). Considering the remineralizing solution, RMGIC presented higher fluoride release rate, followed by GIC, while RC did not release any fluoride. Yet for the demineralizing solution, RMGIC and GIC released similar fluoride rates, overcoming RC (p <.05). Micrographs revealed no changes on the restorations margins, although enamel detachment was observed for RC and GIC after pH-cycling.

Original languageEnglish
JournalMicroscopy Research and Technique
DOIs
StateAccepted/In press - 2021

Bibliographical note

Funding Information:
The authors thank the following programs, institutions, and company for having supported this work: Center for Laser and Applications, Nuclear and Energy Research Institute (IPEN), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES/PROEX; Grant No 3110/2010), São Paulo Research Foundation (FAPESP; Grant No 2012/02651‐6), SAE/UNICAMP (Grant No 01‐P‐164/2014), and Microdont (São Paulo, Brazil).

Publisher Copyright:
© 2021 Wiley Periodicals LLC.

Keywords

  • dental enamel
  • dental materials
  • enamel demineralization
  • optical coherence tomography
  • scanning electron microscopy

Fingerprint

Dive into the research topics of 'Analysis of enamel/restoration interface submitted cariogenic challenge and fluoride release'. Together they form a unique fingerprint.

Cite this