TY - JOUR
T1 - Aislación y Caracterización de Células Madre Mesenquimales Provenientes de Pulpa y Folículo Dentario Humano
AU - Brizuela C., Claudia
AU - Galleguillos G., Sussy
AU - Carrión A., Flavio
AU - Cabrera P., Carolina
AU - Luz C., Patricia
AU - Inostroza S., Carolina
PY - 2013/6
Y1 - 2013/6
N2 - It was recently discovered that dental tissues are important sources of mesenchymal stem cells (MSCs). In the oral cavity MSCs can be found in the pulp, dental follicle, apical papilla and gingival tissue, among others. Many studies support the therapeutic potential of MSCs in regenerative therapies. The objective of this study was to isolate and culture mesenchymal stem cells from human dental pulp and follicle, and to characterize their immunophenotype and differentiation potential to adipogenic, chondrogenic and osteogenic lineages. Oral cavity stem cells were cultured from pulp and dental follicle of wisdom teeth from young permanent teeth. Immunotypification of MSCs was performed by flow cytometry and cultures were evaluated for their ability to differentiate into the three lineages mentioned. Our results corroborate that cultured oral MSC cells isolated from pulp and dental follicle were mesenchymal in origin, being the latter more easy to obtain. Dental follicle MSCs have greater growth potential and differentiation capacity compared to dental pulp MSCs, probably due to their more immature developmental state.
AB - It was recently discovered that dental tissues are important sources of mesenchymal stem cells (MSCs). In the oral cavity MSCs can be found in the pulp, dental follicle, apical papilla and gingival tissue, among others. Many studies support the therapeutic potential of MSCs in regenerative therapies. The objective of this study was to isolate and culture mesenchymal stem cells from human dental pulp and follicle, and to characterize their immunophenotype and differentiation potential to adipogenic, chondrogenic and osteogenic lineages. Oral cavity stem cells were cultured from pulp and dental follicle of wisdom teeth from young permanent teeth. Immunotypification of MSCs was performed by flow cytometry and cultures were evaluated for their ability to differentiate into the three lineages mentioned. Our results corroborate that cultured oral MSC cells isolated from pulp and dental follicle were mesenchymal in origin, being the latter more easy to obtain. Dental follicle MSCs have greater growth potential and differentiation capacity compared to dental pulp MSCs, probably due to their more immature developmental state.
KW - Dental pulp
KW - Follicle
KW - Mesenchymal stem cells
KW - Multilineage differentiation
KW - Proliferation potential
KW - Células madre mesenquimales
KW - Pulpa y folículo dental
KW - Diferenciación multilinaje
KW - Potencial de proliferación
UR - http://www.scopus.com/inward/record.url?scp=84884773750&partnerID=8YFLogxK
U2 - 10.4067/S0717-95022013000200063
DO - 10.4067/S0717-95022013000200063
M3 - Article
AN - SCOPUS:84884773750
SN - 0717-9367
VL - 31
SP - 739
EP - 746
JO - International Journal of Morphology
JF - International Journal of Morphology
IS - 2
ER -