Active mmp-8 quantitative test as an adjunctive tool for early diagnosis of periodontitis

Marcela Hernández, Mauricio Baeza, Ismo T. Räisänen, Johanna Contreras, Taina Tervahartiala, Alejandra Chaparro, Timo Sorsa, Patricia Hernández-Ríos

Research output: Contribution to journalArticlepeer-review

Abstract

Periodontitis is a host-mediated bacterial disease that affects the tooth attachment apparatus. Metalloproteinase-8 (MMP-8), a validated biomarker, could aid in clinical diagnosis. This study aimed to evaluate the diagnostic performance of active (a) MMP-8 immunotest versus total (t) MMP-8 ELISA for quantitative real-time diagnosis and assessment of periodontitis severity at the site level. Gingival crevicular fluid (GCF) was sampled from 30 healthy, 42 mild, and 59 severe periodontitis sites from thirty-one volunteers. MMP-8 concentrations were determined by time-resolved immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using the STATA package. Both active and total MMP-8-based methods discriminated among sites according to periodontal diagnosis and severity, with a positive correlation between the two tests (p < 0.001). (a) MMP-8 models showed the best performance in receiver operating characteristic (ROC) curves to discriminate between healthy and periodontitis sites (area under the curve [AUC] = 0.89), while (t) MMP-8 demonstrated a high diagnostic precision in the detection of mild from severe periodontitis sites (AUC ≥ 0.80). The use of (a) MMP-8 and (t) MMP-8 could represent a useful adjunctive tool for periodontitis diagnosis and severity. These results support the applicability of new point-of-care methods in the monitoring of high-risk periodontal patients.

Original languageEnglish
Article number1503
JournalDiagnostics
Volume11
Issue number8
DOIs
StatePublished - Aug 2021

Bibliographical note

Funding Information:
Funding: This research was funded by National Fund for Scientific and Technological Development (FONDECYT), grant number 1200098, ANID, Chilean government; the Helsinki and Uusimaa Hospital District (HUS) (grant numbers TYH2016251, TYH2017251, TYH2018229, TYH2019319, Y1014SL017, Y1014SL018, Y1014SULE1), Finland; the Finnish Dental Association Apollonia, Finland; the Karolin-ska Institutet, Sweden. Ismo T. Räisänen has received dissertation grants from The Yrjö Jahnsson Foundation sr, The Paulo Foundation, The Emil Aaltonen Foundation sr, The Juhani Aho Foundation for Medical Research sr, The Orion Research Foundation sr, The Finnish Dental Society Apollonia and Helsingin Seudun Hammaslääkärit r.y. (Dentists of Helsinki Region Association), Finland.

Funding Information:
This research was funded by National Fund for Scientific and Technological Development (FONDECYT), grant number 1200098, ANID, Chilean government; the Helsinki and Uusimaa Hospital District (HUS) (grant numbers TYH2016251, TYH2017251, TYH2018229, TYH2019319, Y1014SL017, Y1014SL018, Y1014SULE1), Finland; the Finnish Dental Association Apollonia, Finland; the Karolin-ska Institutet, Sweden. Ismo T. R?is?nen has received dissertation grants from The Yrj? Jahnsson Foundation sr, The Paulo Foundation, The Emil Aaltonen Foundation sr, The Juhani Aho Foundation for Medical Research sr, The Orion Research Foundation sr, The Finnish Dental Society Apollonia and Helsingin Seudun Hammasl??k?rit r.y. (Dentists of Helsinki Region Association), Finland.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Biomarkers
  • Diagnosis
  • Gingival crevicular fluid
  • Matrix metalloproteinase-8
  • Periodontal disease
  • Periodontitis

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