A universal dental adhesive containing copper nanoparticles stabilizes the hybrid layer in eroded dentin after 1 year

Taíse Alessandra Hanzen; Mario Felipe Gutiérrez; Thalita de Paris Matos; Alexandra Mara de Paula; Fabiana Suelen Figueredo de Siqueira; Andrés Felipe Millán Cardenas; Andres Viteri-Garcia; Marcela Hernandez; Alessandro D. Loguercio; Alessandra Reis

doi:10.1016/j.ijadhadh.2021.103041

A universal dental adhesive containing copper nanoparticles stabilizes the hybrid layer in eroded dentin after 1 year

Taíse Alessandra Hanzen, Mario Felipe Gutiérrez, Thalita de Paris Matos, Alexandra Mara de Paula, Fabiana Suelen Figueredo de Siqueira, Andrés Felipe Millán Cardenas, Andres Viteri-Garcia, Marcela Hernandez, Alessandro D. Loguercio^*, Alessandra Reis

^*Corresponding author for this work

Facultad de Odontología

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

This study evaluated the matrix metalloproteases (MMP) inhibition of the copper nanoparticles (CuNp), and the effects of their addition into a universal adhesive system on antimicrobial activity (AMA), ultimate tensile strength (UTS), water sorption (WS), solubility (SO), as well as the 24h and 1y microtensile bond strength (μTBS), nanoleakage (NL) and in situ degree of conversion (DC), on sound and eroded dentin. Anti-MMP activity was evaluated for MMP-2, -8 and -9. CuNp (0%[control] and 0.1 wt%) were added into Ambar Universal. Antimicrobial activity against S. mutans was tested using agar diffusion. For UTS, specimens were tested after 24h and 28d. WS and SO were evaluated during 56d. After induced erosion, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks, which were used for evaluation of μTBS and nanoleakage at the 24h and 1y periods. DC was evaluated at the 24h period in these beam-like specimens. Data were submitted to appropriate statistical analyses (α = 0.05). CuNp demonstrated anti MMP-activity (p < 0.05) besides providing antimicrobial activity (p < 0.05), as well as maintained stable μTBS and NL values over 1-year, for both substrates (p < 0.05). Thus, CuNp may prevent the degradation of the adhesive interface, mainly in eroded dentin. Clinical relevance: Copper-containing universal adhesives could be considered a feasible alternative to improve adhesive properties to sound and eroded dentin after 1-year.

Original language	English
Article number	103041
Pages (from-to)	1-10
Number of pages	10
Journal	International Journal of Adhesion and Adhesives
Volume	113
Early online date	11 Nov 2021
DOIs	https://doi.org/10.1016/j.ijadhadh.2021.103041
State	Published - Mar 2022

Bibliographical note

Funding Information:
This study was partially supported by the National Council for Scientific and Technological Development (CNPq) , under grants 303332/2017–4 and 305588/2014–1 and the Coordination of Improvement of Higher-Level Personnel ( CAPES ).

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

Copper
Degree of conversion
Microtensile bond strength
Nanoleakage
Nanoparticles
Universal adhesive system

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10.1016/j.ijadhadh.2021.103041

Cite this

Hanzen, T. A., Gutiérrez, M. F., de Paris Matos, T., Mara de Paula, A., Figueredo de Siqueira, F. S., Millán Cardenas, A. F., Viteri-Garcia, A., Hernandez, M., Loguercio, A. D., & Reis, A. (2022). A universal dental adhesive containing copper nanoparticles stabilizes the hybrid layer in eroded dentin after 1 year. International Journal of Adhesion and Adhesives, 113, 1-10. Article 103041. https://doi.org/10.1016/j.ijadhadh.2021.103041

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title = "A universal dental adhesive containing copper nanoparticles stabilizes the hybrid layer in eroded dentin after 1 year",

abstract = "This study evaluated the matrix metalloproteases (MMP) inhibition of the copper nanoparticles (CuNp), and the effects of their addition into a universal adhesive system on antimicrobial activity (AMA), ultimate tensile strength (UTS), water sorption (WS), solubility (SO), as well as the 24h and 1y microtensile bond strength (μTBS), nanoleakage (NL) and in situ degree of conversion (DC), on sound and eroded dentin. Anti-MMP activity was evaluated for MMP-2, -8 and -9. CuNp (0%[control] and 0.1 wt%) were added into Ambar Universal. Antimicrobial activity against S. mutans was tested using agar diffusion. For UTS, specimens were tested after 24h and 28d. WS and SO were evaluated during 56d. After induced erosion, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks, which were used for evaluation of μTBS and nanoleakage at the 24h and 1y periods. DC was evaluated at the 24h period in these beam-like specimens. Data were submitted to appropriate statistical analyses (α = 0.05). CuNp demonstrated anti MMP-activity (p < 0.05) besides providing antimicrobial activity (p < 0.05), as well as maintained stable μTBS and NL values over 1-year, for both substrates (p < 0.05). Thus, CuNp may prevent the degradation of the adhesive interface, mainly in eroded dentin. Clinical relevance: Copper-containing universal adhesives could be considered a feasible alternative to improve adhesive properties to sound and eroded dentin after 1-year.",

keywords = "Copper, Degree of conversion, Microtensile bond strength, Nanoleakage, Nanoparticles, Universal adhesive system",

author = "Hanzen, {Ta{\'i}se Alessandra} and Guti{\'e}rrez, {Mario Felipe} and {de Paris Matos}, Thalita and {Mara de Paula}, Alexandra and {Figueredo de Siqueira}, {Fabiana Suelen} and {Mill{\'a}n Cardenas}, {Andr{\'e}s Felipe} and Andres Viteri-Garcia and Marcela Hernandez and Loguercio, {Alessandro D.} and Alessandra Reis",

note = "Funding Information: This study was partially supported by the National Council for Scientific and Technological Development (CNPq) , under grants 303332/2017–4 and 305588/2014–1 and the Coordination of Improvement of Higher-Level Personnel ( CAPES ). Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

year = "2022",

month = mar,

doi = "10.1016/j.ijadhadh.2021.103041",

language = "English",

volume = "113",

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Hanzen, TA, Gutiérrez, MF, de Paris Matos, T, Mara de Paula, A, Figueredo de Siqueira, FS, Millán Cardenas, AF, Viteri-Garcia, A, Hernandez, M, Loguercio, AD & Reis, A 2022, 'A universal dental adhesive containing copper nanoparticles stabilizes the hybrid layer in eroded dentin after 1 year', International Journal of Adhesion and Adhesives, vol. 113, 103041, pp. 1-10. https://doi.org/10.1016/j.ijadhadh.2021.103041

TY - JOUR

T1 - A universal dental adhesive containing copper nanoparticles stabilizes the hybrid layer in eroded dentin after 1 year

AU - Hanzen, Taíse Alessandra

AU - Gutiérrez, Mario Felipe

AU - de Paris Matos, Thalita

AU - Mara de Paula, Alexandra

AU - Figueredo de Siqueira, Fabiana Suelen

AU - Millán Cardenas, Andrés Felipe

AU - Viteri-Garcia, Andres

AU - Hernandez, Marcela

AU - Loguercio, Alessandro D.

AU - Reis, Alessandra

N1 - Funding Information: This study was partially supported by the National Council for Scientific and Technological Development (CNPq) , under grants 303332/2017–4 and 305588/2014–1 and the Coordination of Improvement of Higher-Level Personnel ( CAPES ). Publisher Copyright: © 2021 Elsevier Ltd

PY - 2022/3

Y1 - 2022/3

N2 - This study evaluated the matrix metalloproteases (MMP) inhibition of the copper nanoparticles (CuNp), and the effects of their addition into a universal adhesive system on antimicrobial activity (AMA), ultimate tensile strength (UTS), water sorption (WS), solubility (SO), as well as the 24h and 1y microtensile bond strength (μTBS), nanoleakage (NL) and in situ degree of conversion (DC), on sound and eroded dentin. Anti-MMP activity was evaluated for MMP-2, -8 and -9. CuNp (0%[control] and 0.1 wt%) were added into Ambar Universal. Antimicrobial activity against S. mutans was tested using agar diffusion. For UTS, specimens were tested after 24h and 28d. WS and SO were evaluated during 56d. After induced erosion, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks, which were used for evaluation of μTBS and nanoleakage at the 24h and 1y periods. DC was evaluated at the 24h period in these beam-like specimens. Data were submitted to appropriate statistical analyses (α = 0.05). CuNp demonstrated anti MMP-activity (p < 0.05) besides providing antimicrobial activity (p < 0.05), as well as maintained stable μTBS and NL values over 1-year, for both substrates (p < 0.05). Thus, CuNp may prevent the degradation of the adhesive interface, mainly in eroded dentin. Clinical relevance: Copper-containing universal adhesives could be considered a feasible alternative to improve adhesive properties to sound and eroded dentin after 1-year.

AB - This study evaluated the matrix metalloproteases (MMP) inhibition of the copper nanoparticles (CuNp), and the effects of their addition into a universal adhesive system on antimicrobial activity (AMA), ultimate tensile strength (UTS), water sorption (WS), solubility (SO), as well as the 24h and 1y microtensile bond strength (μTBS), nanoleakage (NL) and in situ degree of conversion (DC), on sound and eroded dentin. Anti-MMP activity was evaluated for MMP-2, -8 and -9. CuNp (0%[control] and 0.1 wt%) were added into Ambar Universal. Antimicrobial activity against S. mutans was tested using agar diffusion. For UTS, specimens were tested after 24h and 28d. WS and SO were evaluated during 56d. After induced erosion, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks, which were used for evaluation of μTBS and nanoleakage at the 24h and 1y periods. DC was evaluated at the 24h period in these beam-like specimens. Data were submitted to appropriate statistical analyses (α = 0.05). CuNp demonstrated anti MMP-activity (p < 0.05) besides providing antimicrobial activity (p < 0.05), as well as maintained stable μTBS and NL values over 1-year, for both substrates (p < 0.05). Thus, CuNp may prevent the degradation of the adhesive interface, mainly in eroded dentin. Clinical relevance: Copper-containing universal adhesives could be considered a feasible alternative to improve adhesive properties to sound and eroded dentin after 1-year.

KW - Copper

KW - Degree of conversion

KW - Microtensile bond strength

KW - Nanoleakage

KW - Nanoparticles

KW - Universal adhesive system

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DO - 10.1016/j.ijadhadh.2021.103041

M3 - Article

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VL - 113

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JO - International Journal of Adhesion and Adhesives

JF - International Journal of Adhesion and Adhesives

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A universal dental adhesive containing copper nanoparticles stabilizes the hybrid layer in eroded dentin after 1 year

Abstract

Bibliographical note

Keywords

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