Abstract
The effect of an early single injection of biodegradable core-shell nanoparticles (NPs) loaded with various low doses of recombinant human bone morphogenetic protein-7 (rhBMP-7/rhOP-1) on new bone regeneration and consolidation in a rabbit model of tibial distraction osteogenesis (DO) was investigated. The Regenerate bone was examined using soft radiography, densitometry, micro-computed tomography and histomorphometry. Compared to control, higher bone fill scores and a two- to three-fold increase in the quantity of mineralized tissue were prominent in the 1.0 and 5.0 μg OP-1/NPs groups, 3 weeks post-injections (P>0.05). Histologically, the distraction gap was completely ossified and the osteotomy margins poorly demarcated in those groups, one week into the consolidation phase. An up-regulation of various growth factors, ligands, and receptors was observed using immunohistochemistry. This novel hybrid delivery system maintains the bioactivity of the encapsulant, minimizes the therapeutic doses of rhOP-1, and accelerates DO via its localized, release-controlled, osteogenic, and naturally biocompatible polymeric properties.
| Original language | English |
|---|---|
| Pages (from-to) | 44-55 |
| Number of pages | 12 |
| Journal | Growth Factors |
| Volume | 28 |
| Issue number | 1 |
| Early online date | 1 Dec 2009 |
| DOIs | |
| State | Published - Feb 2010 |
| Externally published | Yes |
Keywords
- Bone densitometry
- Delivery systems
- Distraction osteogenesis
- Histomorphometry
- Nanoparticles
- Osteogenic protein-1