A hybrid rhOP-1 delivery system enhances new bone regeneration and consolidation in a rabbit model of distraction osteogenesis

Ziyad S. Haidar; Maryam Tabrizian; Reggie C. Hamdy

doi:10.3109/08977190903367788

A hybrid rhOP-1 delivery system enhances new bone regeneration and consolidation in a rabbit model of distraction osteogenesis

Ziyad S. Haidar, Maryam Tabrizian, Reggie C. Hamdy

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The effect of an early single injection of biodegradable core-shell nanoparticles (NPs) loaded with various low doses of recombinant human bone morphogenetic protein-7 (rhBMP-7/rhOP-1) on new bone regeneration and consolidation in a rabbit model of tibial distraction osteogenesis (DO) was investigated. The Regenerate bone was examined using soft radiography, densitometry, micro-computed tomography and histomorphometry. Compared to control, higher bone fill scores and a two- to three-fold increase in the quantity of mineralized tissue were prominent in the 1.0 and 5.0 μg OP-1/NPs groups, 3 weeks post-injections (P>0.05). Histologically, the distraction gap was completely ossified and the osteotomy margins poorly demarcated in those groups, one week into the consolidation phase. An up-regulation of various growth factors, ligands, and receptors was observed using immunohistochemistry. This novel hybrid delivery system maintains the bioactivity of the encapsulant, minimizes the therapeutic doses of rhOP-1, and accelerates DO via its localized, release-controlled, osteogenic, and naturally biocompatible polymeric properties.

Original language	English
Pages (from-to)	44-55
Number of pages	12
Journal	Growth Factors
Volume	28
Issue number	1
DOIs	https://doi.org/10.3109/08977190903367788
State	Published - Feb 2010
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank Dr Fereshteh Azari for her assistance with the cryo-TEM and Mrs Maria Kotsiopriftis and Mrs Dominique Lauzier for their assistance in histomorphometry and immunohistochemistry. This work was supported by an operating grant from the Shriners of North America, Fonds de la Recherche en Santé du Québec, the National Science and Engineering Research Council (NSERC), the Canadian Institutes of Health Research (CIHR) Regenerative Medicine/Nanomedicine, and the Center for Biorecognition and Biosensors (CBB), McGill University, Montréal, Québec, Canada. Dr Haidar acknowledges scholarships from the Center for Bone and Periodontal Diseases Research and the Shriners Hospital, Montréal, Québec, Canada.

Keywords

Bone densitometry
Delivery systems
Distraction osteogenesis
Histomorphometry
Nanoparticles
Osteogenic protein-1

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title = "A hybrid rhOP-1 delivery system enhances new bone regeneration and consolidation in a rabbit model of distraction osteogenesis",

abstract = "The effect of an early single injection of biodegradable core-shell nanoparticles (NPs) loaded with various low doses of recombinant human bone morphogenetic protein-7 (rhBMP-7/rhOP-1) on new bone regeneration and consolidation in a rabbit model of tibial distraction osteogenesis (DO) was investigated. The Regenerate bone was examined using soft radiography, densitometry, micro-computed tomography and histomorphometry. Compared to control, higher bone fill scores and a two- to three-fold increase in the quantity of mineralized tissue were prominent in the 1.0 and 5.0 μg OP-1/NPs groups, 3 weeks post-injections (P>0.05). Histologically, the distraction gap was completely ossified and the osteotomy margins poorly demarcated in those groups, one week into the consolidation phase. An up-regulation of various growth factors, ligands, and receptors was observed using immunohistochemistry. This novel hybrid delivery system maintains the bioactivity of the encapsulant, minimizes the therapeutic doses of rhOP-1, and accelerates DO via its localized, release-controlled, osteogenic, and naturally biocompatible polymeric properties.",

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author = "Haidar, {Ziyad S.} and Maryam Tabrizian and Hamdy, {Reggie C.}",

note = "Funding Information: The authors would like to thank Dr Fereshteh Azari for her assistance with the cryo-TEM and Mrs Maria Kotsiopriftis and Mrs Dominique Lauzier for their assistance in histomorphometry and immunohistochemistry. This work was supported by an operating grant from the Shriners of North America, Fonds de la Recherche en Sant{\'e} du Qu{\'e}bec, the National Science and Engineering Research Council (NSERC), the Canadian Institutes of Health Research (CIHR) Regenerative Medicine/Nanomedicine, and the Center for Biorecognition and Biosensors (CBB), McGill University, Montr{\'e}al, Qu{\'e}bec, Canada. Dr Haidar acknowledges scholarships from the Center for Bone and Periodontal Diseases Research and the Shriners Hospital, Montr{\'e}al, Qu{\'e}bec, Canada.",

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N2 - The effect of an early single injection of biodegradable core-shell nanoparticles (NPs) loaded with various low doses of recombinant human bone morphogenetic protein-7 (rhBMP-7/rhOP-1) on new bone regeneration and consolidation in a rabbit model of tibial distraction osteogenesis (DO) was investigated. The Regenerate bone was examined using soft radiography, densitometry, micro-computed tomography and histomorphometry. Compared to control, higher bone fill scores and a two- to three-fold increase in the quantity of mineralized tissue were prominent in the 1.0 and 5.0 μg OP-1/NPs groups, 3 weeks post-injections (P>0.05). Histologically, the distraction gap was completely ossified and the osteotomy margins poorly demarcated in those groups, one week into the consolidation phase. An up-regulation of various growth factors, ligands, and receptors was observed using immunohistochemistry. This novel hybrid delivery system maintains the bioactivity of the encapsulant, minimizes the therapeutic doses of rhOP-1, and accelerates DO via its localized, release-controlled, osteogenic, and naturally biocompatible polymeric properties.

AB - The effect of an early single injection of biodegradable core-shell nanoparticles (NPs) loaded with various low doses of recombinant human bone morphogenetic protein-7 (rhBMP-7/rhOP-1) on new bone regeneration and consolidation in a rabbit model of tibial distraction osteogenesis (DO) was investigated. The Regenerate bone was examined using soft radiography, densitometry, micro-computed tomography and histomorphometry. Compared to control, higher bone fill scores and a two- to three-fold increase in the quantity of mineralized tissue were prominent in the 1.0 and 5.0 μg OP-1/NPs groups, 3 weeks post-injections (P>0.05). Histologically, the distraction gap was completely ossified and the osteotomy margins poorly demarcated in those groups, one week into the consolidation phase. An up-regulation of various growth factors, ligands, and receptors was observed using immunohistochemistry. This novel hybrid delivery system maintains the bioactivity of the encapsulant, minimizes the therapeutic doses of rhOP-1, and accelerates DO via its localized, release-controlled, osteogenic, and naturally biocompatible polymeric properties.

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A hybrid rhOP-1 delivery system enhances new bone regeneration and consolidation in a rabbit model of distraction osteogenesis

Abstract

Bibliographical note

Keywords

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