Regulation of gene expression has been involved in long-term memory consolidation. Present results support the role of Rel/NFκ-B like activation in this process. In the crab Chasmagnathus, the spaced presentation of 15 or more danger stimuli induces long-term habituation (LTH), while no LTH is observed after a massed training of 600 trials. When a group trained with 30 spaced trials was compared with a passive control group and massed trained groups, a higher level of specific Rel/κ-B like DNA-binding activity was found in brain nuclear extracts. These results strongly suggest that the enhancement of Rel/κ-B like DNA-binding activity in the brain is specifically related to LTH formation.
Bibliographical noteFunding Information:
We would especially like to thank Alberto Kornblitth for his support and helpful discussion and comments on the manuscript. We would also like to thank Angel Vidal for technical assistance. This work was supported by CONICET-Argentina and University of Buenos Aires grants.
- Gel shift
- Long-term memory
- Transcription factors